Biozatix News – Informasi Sains Teknologi dan Kesehatan
Cell Dissociation Reagents: Accutase
| Accutase is a ready to use cell detachment solution, which is non-mammalian, non-bacterial replacement for all applications of trypsin. |  |
Features
- Gentle and efficient dissociation of any adherent cell line
- No mammalian or bacterial components are contained
- No neutralization steps by serum or trypsin inhibitors are required
- Works extremely well on embryonic and neuronal stem cells
Cell Lines Cryopreserved with Accutase™
| ・hESCs ・vascular endothelial cells ・hepatocyte progenitors ・adherent CHO cells ・293 cells ・3T3 ・HeLa ・M24 metastatic melanoma ・gliomas D54 ・fibroblasts ・hepatocytes ・primary chick embryo neuronal cells ・adherent BHK cells ・L929 cells |
・Vero ・NT2 ・A375 metastatic melanoma ・HT1080 fibrosarcoma cells ・keratinocytes ・vascular smooth muscle cells ・bone marrow stem cells ・macrophages ・immortalized mouse testicular germ cells ・COS ・MG63 ・gliomas U251 ・Sf9 insect cells |
Application
Human MG63 Fibrosarcoma cells cultured in DMEM + 10% FBS were treated with Accutase. Treatment resulted in rapid cell detachment, a single cell suspension, and high viability. Accutase is gentle on cells; viability was 97 ± 3% even after 45 minutes in Accutase.
Cell Dissociation Reagents: Accutase