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Description
The FavorPrep™ Blood Genomic DNA Extraction Kit is designed for rapid extraction of pure genomic DNA from whole blood, serum, buffy coat, body fluids, lymphocytes and cultured cell. As a column-type tube is utilized in the purification process in three simple steps of binding, washing and then elution for the safe and convenient extraction of high-purity genomic DNA.
Features
- Extraction of genomic DNA from whole blood, plasma, serum, buffy coat, body fluids, lymphocytes and cultured cells.
- Purified DNA is ready for downstream application such as PCR, Southern blotting.
- Centrifugation-based method.
- Efficiently remove cellular debair and inhibitors
- No phenol/chloroform extraction and ethanol precipitation required.
Applications
- PCR
- Southern Blotting
- Medicolegal Analysis
Sampling
Up to 200 μl of whole blood (with anti-coagulant), plasma, serum, buffy coat, or body fluids; up to 5x105lymphocytes or cultured cells in 200μl PBS.
Yield
About 5μg of total DNA from 200μl of human whole blood; up to 50μg of total DNA, depends on the sample types and the number of cells in the sample.
| Yield of Purification | ||
| Sample | Amounts | Yield |
| Whole Blood | 200 μl | 3~6μg |
| Whole Blood | 1 ml | 10~40μg |
| Buffy Coat | 200 μl | 30~60μg |
| Cultured Cells | 5*106 | 15 ~30μg |
| Bacterial Cells | 2*109 | 100~300μg |
| The yield of genomic DNA is variable depending on the sample types. | ||
Handling Time
About 60 minutes depending upon the sample types.
