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HPLC COSMOCORE 2.6Cholester
- Cholesterol-bonded reversed-phase core-shell column
- Usable under the same conditions as C18 columns
- Better selectivity for cis-trans isomers, polyphenols, and natural products
About Core-Shell 2.6Cholester Particles
COSMOCORE 2.6Cholester is packed with cholesterol-bonded 2.6 µm core-shell particles (Figure 1 and 2). It can achieve low pressure compared to fully-porous sub-2 µm columns (Figure 3).
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| Figure 1: Core-Shell Particle | Figure 2: Bonded Cholestrol Stationary Phase |
Figure 3: Comparison of Column Pressure
Column size 2.1mmI.D.-100mm, Mobile Phase Acetonitrile/Water=70/30, Temperature: 40°C
Comparison with C18
COSMOCORE 2.6Cholester offers improved separation for cis-trans isomers than C18 under typical reversed-phase mobile phase.
| Condition | ||||
|---|---|---|---|---|
| Column size | 2.1 mm I.D. – 100 mm | Sample |
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| Mobile phase | Methanol | |||
| Flow rate | 0.4 ml/min | |||
| Temperature | 40°C | Inj. Vol | 0.5µl | |
| Detection | UV280nm |  |
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Separation Properties
COSMOCORE 2.6Cholester has about the same hydrophobicity as C18. It is not necessary to change the analytical conditions when replacing C18 Columns with COSMOCORE 2.6Cholester.
| Condition | ||||
|---|---|---|---|---|
| Column size | 2.1 mm I.D. – 100 mm | Sample |
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| Mobile phase | Acetonitrile/ H2O = 50/50 | |||
| Flow rate | 0.4 ml/min | |||
| Temperature | 40°C | |||
| Detection | UV254nm | Inj. Vol | 0.25µl | |
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