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Tabel dibawah ini akan membantu anda untuk menentukan jenis column HPLC yang anda butuhkan.
| Sample | Separation mode | Packing material | USP Code | Stationary phase |
Special features and applications |
|---|---|---|---|---|---|
| Organic compounds (low M.W.) |
Reversed phase |
C18-MS-II | L1 | Octadecyl group |
– Multi-purpose C18?column – Monofunctional silylation on ultra-pure silica gel for separation of the widest range of compounds |
| C18-AR-II | L1 | -Features strong acid resistance and suitable for acidic compounds and peptides | |||
| C18-PAQ | L1 | – Reversed phase column, compatible with 100% water based mobile phase. – Suitable for hydrophilic compounds |
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| Cholester | – | cholesteryl group |
– Usable under the same condition as C18 – Unique rigid cholesteryl structure improves separation |
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| πNAP | – | naphtylethyl group |
– Stronger π-π interaction than Phenyl column | ||
| PYE | – | pyrenylethyl group |
– Most powerful π-π interaction | ||
| NPE | – | nitrophenylethyl group |
– Separation utilizing π-π interaction and dipole-dipole interaction | ||
| PBB-R | – | pentabromobenzyl group |
– Separation utilizing dispersion force | ||
| C22-AR-II | – | docosyl group |
– Alkyl chain columns except C18column | ||
| C8-MS | L7 | octyl group |
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| C4-MS | L26 | butyl group |
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| TMS-MS | L13 | trimethyl group |
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| PE-MS | L11 | phenylethyl group |
– π-π interaction | ||
| CN-MS | L10 | cyanopropyl group |
– Great reproducibility using isocratic elution mode | ||
| Normal phase |
SL-II | L3 | – | – Suitable for preparative separation | |
| Hydrophilic interaction |
HILIC | – | triazole | – Retains highly polar compounds that would not be retained in C18?column | |
| Monosaccharides Oligosaccharides |
Hydrophilic interaction |
Sugar-D | – | secondary/tertiary amine | – Suitable for monosaccharides, oligosaccharides |
| NH2-MS | – | aminopropyl group |
– Primary amine bonded column | ||
| Proteins | Reversed phase |
Protein-R | – | octadecyl group |
– Provides improved separation, high recovery rate, and acid resistance |
| C18-AR-300 | L1 | octadecyl group |
– Wide pore type reversed phase columns | ||
| C8-AR-300 | L7 | octyl group |
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| C4-AR-300 | L26 | butyl group |
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| Ph-AR-300 | L11 | phenyl group |
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| Proteins | Gel permeation |
Diol-120-II | L20 | diol group | – Silica-based gel filtration column Selection of pore size: 5,000-100,000 |
| Diol-300-II | L20 | – Silica-based gel filtration column Selection of pore size: 10,000-700,000 |
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| Ion-exchange | DEAE | – | Diethylaminoethyl type |
– Weak anion-exchange | |
| QA | L23 | quarternary ammonium type |
– Strong anion-exchange | ||
| CM | – | carboxymethyl type |
– Weak cation-exchange | ||
| SP | – | sulfopropyl type |
– Strong cation-exchange | ||
| Hydrophobic interaction |
HIC | – | – | – Suitable for separation of peptide, and there is only a little less in enzyme activity and the teriary structure of proteins | |
| Fullerenes | – | Buckyprep | – | pyrenylpropyl group |
– Standard column for fullerene separation |
| Buckyprep-M | – | phenothiazinyl group |
– Designed to separate metallofullerenes | ||
| PBB | – | pentabromobenzyl group |
– Designed for preparative separation of C60, C70 | ||
| PYE | – | pyrenylethyl group |
– Separation of Fullerenes and structural isomers | ||
| NPE | – | nitrophenylethyl group |
– Separation of derivatived fullerene | ||
| Carbon nanotubes |
Gel permeation |
CNT | hydrophilic group |
– separation of carbon nanotubes |
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Tabel bantuan menentukan column HPLC