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Description
The Blood Cell Genomic DNA Extraction blood Maxi Kit is designed for purification of total DNA (including genomic, mitochondrial and viral DNA) from large volumes of blood sample or cultured cells using a safe protocol that eliminates the use of organic solvents such as phenol and chloroform. By using proteinase K and lysis buffer, the kits effectively lyse cells and degrade protein. Purified DNA with approximately 20-30 Kb of size is suitable for PCR or other enzymatic reactions.
Features
- High Purity: DNA is immediately suitable for a variety of applications, including amplification, digestion, PCR etc.
- High Speed: Rapid speed for the isolation of genomic DNA from blood, within 60 minutes.
- Easy Use: Based on a five-step process, purities genomic DNA without the use of caustic organic compounds.
- Safe Use : The kit uses a spin column tube and removes proteins, nucleases in cell. It is not necessary to treat the sample with harmful organic solvents such as phenol and chloroform.
Applications
- PCR
- Southern Blotting
- Real time PCR
- AFLP
- RFLP
Sampling
Blood, other body fluids and cultured cells. Up to 10ml human whole blood
Yield
About 50μg – 150μg of total DNA from 5ml of human whole blood; up to 100-300μg of total DNA from 10ml of human whole blood, depends on the sample types and the number of cells in the sample.
| Yield of Purification | ||
| Sample | Amounts | Yield |
| Whole Blood | 5 ml | 50 ~150μg |
| Whole Blood | 10 ml | 100 ~300μg |
| The yield of genomic DNA is variable depending on the sample types. | ||
Handling Time
About 60 minutes depending upon the sample types.
Storage Conditions
Stable for 2 year at room temperature. The Proteinase K Powder store at -20℃.